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anti ha magnetic beads  (MedChemExpress)


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    Structured Review

    MedChemExpress anti ha magnetic beads
    Anti Ha Magnetic Beads, supplied by MedChemExpress, used in various techniques. Bioz Stars score: 95/100, based on 91 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/anti+ha+magnetic+beads/Anti-HA+Magnetic+Beads/pmc13392861-39-0-4
    Average 95 stars, based on 91 article reviews
    anti ha magnetic beads - by Bioz Stars, 2026-09
    95/100 stars

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    Incubation:

    Article Title: The GSK2-OsHHO3 module antagonistically regulates disease resistance and drought tolerance via interplaying BR and ABA signaling in rice.
    Article Snippet: .. Total proteins were extracted on ice with buffer containing phosphatase inhibitors (04906837001, Roche), then incubated with anti-HA magnetic beads (HY-K0201, MedChemExpress) at 4°C for 2 hours. ..

    Article Title: DDR1 Promotes Immune Evasion in Colorectal Cancer by Orchestrating IL33-Mediated M2-like Polarization of Tumor-Associated Macrophages
    Article Snippet: The lysates were incubated with either anti-DDR1 (#5583, Cell Signaling Technology), anti-DDX21 (#10528-1-AP, Proteintech), or IgG (#3900S, Cell Signaling Technology) at 4°C for 2 hours to form IP complexes, followed by the addition of Dynabeads Protein G (#10003D, Thermo Fisher Scientific) and incubation overnight at 4°C with gentle rotation. .. For Flag- or HA-tagged proteins, lysates were incubated with anti-Flag magnetic beads (# B26101 , Selleck Chemicals) or anti-HA magnetic beads (#HY-K0201, MCE) overnight at 4°C with gentle rotation. ..

    Article Title: Nipah virus matrix protein promotes NF-κB activation by targeting multiple signaling modulators
    Article Snippet: .. At 24 hpt, cells were washed with cold phosphate-buffered saline (PBS) and lysed in lysis buffer (P0013; Beyotime Biotechnology, Shanghai, China) containing protease inhibitor cocktail (HY-K0010; MedChemExpress, Shanghai, China) and PMSF (HY-B0496; MedChemExpress, Shanghai, China) on ice for 10 min. For immunoprecipitation (IP) experiments, the supernatant was then incubated directly with anti-Flag magnetic beads (HY-K0207; MedChemExpress, Shanghai, China) or anti-HA magnetic beads (HY-K0201; MedChemExpress, Shanghai, China) at 4°C overnight with gentle rotation. .. Alternatively, before cell lysis, Myc, V5, or IgG antibodies were incubated with Protein A/G magnetic beads (HY-K0202; MedChemExpress, Shanghai, China) in PBS at 4°C for at least 4 h. The antibody-conjugated beads were washed thrice with PBST (HY-K1022; MedChemExpress, Shanghai, China).

    Magnetic Beads:

    Article Title: The GSK2-OsHHO3 module antagonistically regulates disease resistance and drought tolerance via interplaying BR and ABA signaling in rice.
    Article Snippet: .. Total proteins were extracted on ice with buffer containing phosphatase inhibitors (04906837001, Roche), then incubated with anti-HA magnetic beads (HY-K0201, MedChemExpress) at 4°C for 2 hours. ..

    Article Title: METTL3 Methylation Induces Decay of Endogenous Retroelement Transcripts to Promote Tumor Immune Evasion
    Article Snippet: Cells were routinely tested for Mycoplasma every 3 months using a Mycoplasma qPCR Detection Kit (Beyotime, C0303S) and were confirmed to be Mycoplasma -free. .. Actinomycin D (HY-17559), decitabine (HY-A0004), HLM006474 (HY-16667), 5-FU (HY-90006), Anti-HA Magnetic Beads (HY-K0201), Anti-Flag Magnetic Beads (HY-K0207), and Protein A/G Magnetic Beads (HY-K0202) were purchased from MedChemExpress. .. The following primary antibodies were used: METTL3 (15073-1-AP, 67733-1-Ig; Proteintech; RRID:AB_2142033, RRID:AB_2918502), SETD1A (sc-515590; Santa Cruz Biotechnology, RRID:AB_3719326), E2F4 (10923-1-AP; Proteintech; RRID:AB_2097096), GAPDH (AC033; ABclonal; RRID:AB_2769570), β-actin (66009-1-Ig; Proteintech; RRID:AB_2687938), WDR5 (A3259; ABclonal; RRID:AB_2863033), ASH2L (A4892; ABclonal; RRID:AB_2863377), RbBp5 (A23124; ABclonal; RRID:AB_3719327), lamin B1 (A1910; ABclonal; RRID:AB_2862592), dimethyl-histone H3-K4 (A22143; ABclonal; RRID:AB_3717374), histone H3 (4499; Cell Signaling Technology; RRID:AB_10544537), Flag tag (14793S; Cell Signaling Technology; RRID:AB_2572291), HA tag (51064-2-AP; Proteintech; RRID:AB_11042321), pan methyl lysine (ab7315; Abcam; RRID:AB_305840), pan dimethyl lysine (14117; Cell Signaling Technology; RRID:AB_2798396), N 6 -methyladenosine (m 6 A; ab284130; Abcam; RRID:AB_3717333), RIG-I (3743; Cell Signaling Technology; RRID:AB_2269233), STING (13647; Cell Signaling Technology; RRID:AB_2732796), T-TBK1 (3504; Cell Signaling Technology; RRID:AB_2255663), pTBK1 (5483; Cell Signaling Technology; RRID:AB_10693472, AP1026; ABclonal; RRID:AB_2863910), T-IRF3 (4302; Cell Signaling Technology; RRID:AB_1904036, A19717; ABclonal; RRID:AB_2862750), pIRF3 (79945; Cell Signaling Technology; RRID:AB_2799943, AP0623; ABclonal; RRID:AB_2771210), T-STAT1 (9172; Cell Signaling Technology; RRID:AB_2198300), pSTAT1 (9167; Cell Signaling Technology; RRID:AB_561284, AP0054; ABclonal; RRID:AB_2863803), MDA5 (5321; Cell Signaling Technology; RRID:AB_10694490), CGAS (31659; Cell Signaling Technology; RRID:AB_2799008), and dsRNA-J2 (RNT-SCI-10010; Jena Bioscience; RRID:AB_2922431).

    Article Title: DDR1 Promotes Immune Evasion in Colorectal Cancer by Orchestrating IL33-Mediated M2-like Polarization of Tumor-Associated Macrophages
    Article Snippet: The lysates were incubated with either anti-DDR1 (#5583, Cell Signaling Technology), anti-DDX21 (#10528-1-AP, Proteintech), or IgG (#3900S, Cell Signaling Technology) at 4°C for 2 hours to form IP complexes, followed by the addition of Dynabeads Protein G (#10003D, Thermo Fisher Scientific) and incubation overnight at 4°C with gentle rotation. .. For Flag- or HA-tagged proteins, lysates were incubated with anti-Flag magnetic beads (# B26101 , Selleck Chemicals) or anti-HA magnetic beads (#HY-K0201, MCE) overnight at 4°C with gentle rotation. ..

    Article Title: Oncolytic virus hijacks GOT1 and pyrimidinosomes to fuel pyrimidine synthesis for replication in tumor cells
    Article Snippet: The antibodies used for immunoblotting in this study included monoclonal or polyclonal: anti-β-actin (Proteintech, Cat# 66009-1-Ig), anti-NP (Prepared in our laboratory), anti-S6 ribosomal protein (Cell Signaling Technology [CST], Cat# 2217), anti-phospho-S6 ribosomal protein (S235/236) (CST, Cat# 4858), anti-phospho-p70 S6 Kinase 1 (T389) (ABclonal, Cat# AP0564), anti-p70 S6 Kinase 1 (ABclonal, Cat# A2190), anti-CAD (CST, Cat# 11933), anti-phospho-CAD (S1859) (CST, Cat# 70307), anti-GOT1 (Proteintech, Cat# 14886-1-AP), anti-GOT2 (Proteintech, Cat# 14800-1-AP). anti-UMPS (Proteintech, Cat# 14830-1-AP), anti-DHODH (Proteintech, Cat# 14877-1-AP) and anti-Tom20 (Abcam, Cat# ab283317). .. Anti-Flag Magnetic Beads (Cat# HY-K0207), anti-HA Magnetic Beads (Cat# HY-K0201), Mycophenolate (Cat# HY-B0421), Leflunomide (Cat# HY-B0083), AG 2037 (Cat# HY-14530), Aminooxyacetic acid hemihydrochloride (Cat# HY-107994) were purchased from MedChemExpress (MCE). .. Pyruvate (Cat# S8636), aspartate (Cat# A7219), glutamine (Cat# G7513), glucose (Cat# G8644) were purchased from Sigma-Aldrich.


    Article Title: Nipah virus matrix protein promotes NF-κB activation by targeting multiple signaling modulators
    Article Snippet: .. At 24 hpt, cells were washed with cold phosphate-buffered saline (PBS) and lysed in lysis buffer (P0013; Beyotime Biotechnology, Shanghai, China) containing protease inhibitor cocktail (HY-K0010; MedChemExpress, Shanghai, China) and PMSF (HY-B0496; MedChemExpress, Shanghai, China) on ice for 10 min. For immunoprecipitation (IP) experiments, the supernatant was then incubated directly with anti-Flag magnetic beads (HY-K0207; MedChemExpress, Shanghai, China) or anti-HA magnetic beads (HY-K0201; MedChemExpress, Shanghai, China) at 4°C overnight with gentle rotation. .. Alternatively, before cell lysis, Myc, V5, or IgG antibodies were incubated with Protein A/G magnetic beads (HY-K0202; MedChemExpress, Shanghai, China) in PBS at 4°C for at least 4 h. The antibody-conjugated beads were washed thrice with PBST (HY-K1022; MedChemExpress, Shanghai, China).

    Article Title: KMT5A-mediated methylation of IRF3 promotes tumor progression through immune suppression
    Article Snippet: UNC0379 , MedChemExpress , CAT#HY-12335. .. Anti-HA Magnetic Beads , MedChemExpress , CAT#HY-K0201. .. Anti-Flag magnetic beads , MedChemExpress , CAT#HY-K0207.

    Article Title: HERC4 suppresses colorectal cancer progression by promoting STAT3 ubiquitination
    Article Snippet: CQ , MedChemExpress , CAT:HY-17589A. .. Anti-HA Magnetic Beads , MedChemExpress , CAT: HY-K0201. .. Anti-Flag magnetic beads , MedChemExpress , CAT: HY-K0207.

    Gentle:

    Article Title: DDR1 Promotes Immune Evasion in Colorectal Cancer by Orchestrating IL33-Mediated M2-like Polarization of Tumor-Associated Macrophages
    Article Snippet: The lysates were incubated with either anti-DDR1 (#5583, Cell Signaling Technology), anti-DDX21 (#10528-1-AP, Proteintech), or IgG (#3900S, Cell Signaling Technology) at 4°C for 2 hours to form IP complexes, followed by the addition of Dynabeads Protein G (#10003D, Thermo Fisher Scientific) and incubation overnight at 4°C with gentle rotation. .. For Flag- or HA-tagged proteins, lysates were incubated with anti-Flag magnetic beads (# B26101 , Selleck Chemicals) or anti-HA magnetic beads (#HY-K0201, MCE) overnight at 4°C with gentle rotation. ..

    Article Title: Nipah virus matrix protein promotes NF-κB activation by targeting multiple signaling modulators
    Article Snippet: .. At 24 hpt, cells were washed with cold phosphate-buffered saline (PBS) and lysed in lysis buffer (P0013; Beyotime Biotechnology, Shanghai, China) containing protease inhibitor cocktail (HY-K0010; MedChemExpress, Shanghai, China) and PMSF (HY-B0496; MedChemExpress, Shanghai, China) on ice for 10 min. For immunoprecipitation (IP) experiments, the supernatant was then incubated directly with anti-Flag magnetic beads (HY-K0207; MedChemExpress, Shanghai, China) or anti-HA magnetic beads (HY-K0201; MedChemExpress, Shanghai, China) at 4°C overnight with gentle rotation. .. Alternatively, before cell lysis, Myc, V5, or IgG antibodies were incubated with Protein A/G magnetic beads (HY-K0202; MedChemExpress, Shanghai, China) in PBS at 4°C for at least 4 h. The antibody-conjugated beads were washed thrice with PBST (HY-K1022; MedChemExpress, Shanghai, China).

    Saline:

    Article Title: Nipah virus matrix protein promotes NF-κB activation by targeting multiple signaling modulators
    Article Snippet: .. At 24 hpt, cells were washed with cold phosphate-buffered saline (PBS) and lysed in lysis buffer (P0013; Beyotime Biotechnology, Shanghai, China) containing protease inhibitor cocktail (HY-K0010; MedChemExpress, Shanghai, China) and PMSF (HY-B0496; MedChemExpress, Shanghai, China) on ice for 10 min. For immunoprecipitation (IP) experiments, the supernatant was then incubated directly with anti-Flag magnetic beads (HY-K0207; MedChemExpress, Shanghai, China) or anti-HA magnetic beads (HY-K0201; MedChemExpress, Shanghai, China) at 4°C overnight with gentle rotation. .. Alternatively, before cell lysis, Myc, V5, or IgG antibodies were incubated with Protein A/G magnetic beads (HY-K0202; MedChemExpress, Shanghai, China) in PBS at 4°C for at least 4 h. The antibody-conjugated beads were washed thrice with PBST (HY-K1022; MedChemExpress, Shanghai, China).

    Lysis:

    Article Title: Nipah virus matrix protein promotes NF-κB activation by targeting multiple signaling modulators
    Article Snippet: .. At 24 hpt, cells were washed with cold phosphate-buffered saline (PBS) and lysed in lysis buffer (P0013; Beyotime Biotechnology, Shanghai, China) containing protease inhibitor cocktail (HY-K0010; MedChemExpress, Shanghai, China) and PMSF (HY-B0496; MedChemExpress, Shanghai, China) on ice for 10 min. For immunoprecipitation (IP) experiments, the supernatant was then incubated directly with anti-Flag magnetic beads (HY-K0207; MedChemExpress, Shanghai, China) or anti-HA magnetic beads (HY-K0201; MedChemExpress, Shanghai, China) at 4°C overnight with gentle rotation. .. Alternatively, before cell lysis, Myc, V5, or IgG antibodies were incubated with Protein A/G magnetic beads (HY-K0202; MedChemExpress, Shanghai, China) in PBS at 4°C for at least 4 h. The antibody-conjugated beads were washed thrice with PBST (HY-K1022; MedChemExpress, Shanghai, China).

    Protease Inhibitor:

    Article Title: Nipah virus matrix protein promotes NF-κB activation by targeting multiple signaling modulators
    Article Snippet: .. At 24 hpt, cells were washed with cold phosphate-buffered saline (PBS) and lysed in lysis buffer (P0013; Beyotime Biotechnology, Shanghai, China) containing protease inhibitor cocktail (HY-K0010; MedChemExpress, Shanghai, China) and PMSF (HY-B0496; MedChemExpress, Shanghai, China) on ice for 10 min. For immunoprecipitation (IP) experiments, the supernatant was then incubated directly with anti-Flag magnetic beads (HY-K0207; MedChemExpress, Shanghai, China) or anti-HA magnetic beads (HY-K0201; MedChemExpress, Shanghai, China) at 4°C overnight with gentle rotation. .. Alternatively, before cell lysis, Myc, V5, or IgG antibodies were incubated with Protein A/G magnetic beads (HY-K0202; MedChemExpress, Shanghai, China) in PBS at 4°C for at least 4 h. The antibody-conjugated beads were washed thrice with PBST (HY-K1022; MedChemExpress, Shanghai, China).

    Immunoprecipitation:

    Article Title: Nipah virus matrix protein promotes NF-κB activation by targeting multiple signaling modulators
    Article Snippet: .. At 24 hpt, cells were washed with cold phosphate-buffered saline (PBS) and lysed in lysis buffer (P0013; Beyotime Biotechnology, Shanghai, China) containing protease inhibitor cocktail (HY-K0010; MedChemExpress, Shanghai, China) and PMSF (HY-B0496; MedChemExpress, Shanghai, China) on ice for 10 min. For immunoprecipitation (IP) experiments, the supernatant was then incubated directly with anti-Flag magnetic beads (HY-K0207; MedChemExpress, Shanghai, China) or anti-HA magnetic beads (HY-K0201; MedChemExpress, Shanghai, China) at 4°C overnight with gentle rotation. .. Alternatively, before cell lysis, Myc, V5, or IgG antibodies were incubated with Protein A/G magnetic beads (HY-K0202; MedChemExpress, Shanghai, China) in PBS at 4°C for at least 4 h. The antibody-conjugated beads were washed thrice with PBST (HY-K1022; MedChemExpress, Shanghai, China).



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